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验证数据展示

  • WB analysis using 67880-1-Ig (same clone as 67880-1-PBS)

    WB analysis using 67880-1-Ig (same clone as 67880-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67880-1-Ig (CYPA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67880-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67880-1-Ig (same clone as 67880-1-PBS)

    FC experiment of HeLa using 67880-1-Ig (same clone as 67880-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cyclophilin A (67880-1-Ig, Clone:2B11B9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 67880-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67880-1-Ig (same clone as 67880-1-PBS)

    IF Staining of HeLa using 67880-1-Ig (same clone as 67880-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cyclophilin A antibody (67880-1-Ig, Clone: 2B11B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67880-1-PBS in a different storage buffer formulation.

Cyclophilin A Monoclonal antibody, PBS Only

Cyclophilin A Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 67880-1-PBS

产品说明书

CloneNo. 2B11B9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), ELISA

别名

PPIA, 2B11B9, Cyclosporin A binding protein, Cyclosporin A-binding protein, CYPA

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PPIA Ready-To-Use IHC Kit. PPIA primary antibody included.

产品信息

67880-1-PBS targets Cyclophilin A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1120

Product name: Recombinant human CYPA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-165 aa of BC005320

Sequence: MVNPTVFFDIAVDGEPLGRVSFELFADKVPKTAENFRALSTGEKGFGYKGSCFHRIIPGFMCQGGDFTRHNGTGGKSIYGEKFEDENFILKHTGPGILSMANAGPNTNGSQFFICTAKTEWLDGKHVVFGKVKEGMNIVEAMERFGSRNGKTSKKITIADCGQLE

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 peptidylprolyl isomerase A (cyclophilin A)
别名 PPIA, 2B11B9, Cyclosporin A binding protein, Cyclosporin A-binding protein, CYPA
计算分子量 18 kDa
观测分子量18 kDa
GenBank蛋白编号BC005320
基因名称 PPIA
Gene ID (NCBI) 5478
RRIDAB_2918637
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP62937
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cyclophilin A, also named as PPIA and Rotamase A, belongs to the cyclophilin-type PPIase family and PPIase A subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIA forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. PPIA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication.

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