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Cyclophilin A Monoclonal antibody

Cyclophilin A Monoclonal Antibody for FC, IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

FC, IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B11B9

Cat No : 67880-1-Ig

Print datasheet

Synonyms

Cyclophilin A, Cyclosporin A binding protein, CYPA, CYPH, PPIA, PPIase A, Rotamase A

验证数据展示

  • IF Staining of HeLa using 67880-1-Ig

    IF Staining of HeLa using 67880-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cyclophilin A antibody (67880-1-Ig, Clone: 2B11B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis using 67880-1-Ig

    WB analysis using 67880-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67880-1-Ig (CYPA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • FC experiment of HeLa using 67880-1-Ig

    FC experiment of HeLa using 67880-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cyclophilin A (67880-1-Ig, Clone:2B11B9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PPIA Ready-To-Use IHC Kit. PPIA primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67880-1-Ig targets Cyclophilin A in WB, IF, FC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications FC, IF/ICC, WB, ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen Cyclophilin A fusion protein Ag1120 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name peptidylprolyl isomerase A (cyclophilin A)
Synonyms Cyclophilin A, Cyclosporin A binding protein, CYPA, CYPH, PPIA, PPIase A, Rotamase A
Calculated Molecular Weight 18 kDa
Observed Molecular Weight18 kDa
GenBank Accession NumberBC005320
Gene Symbol PPIA
Gene ID (NCBI) 5478
RRIDAB_2918637
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62937
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclophilin A, also named as PPIA and Rotamase A, belongs to the cyclophilin-type PPIase family and PPIase A subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIA forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. PPIA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication.

实验方案

Product Specific Protocols
WB protocol for Cyclophilin A antibody 67880-1-IgDownload protocol
IF protocol for Cyclophilin A antibody 67880-1-IgDownload protocol
FC protocol for Cyclophilin A antibody 67880-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols