验证数据展示
经过测试的应用
| Positive WB detected in | human plasma |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
HRP-12748 targets Complement factor H in WB applications and shows reactivity with human samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag3482 Product name: Recombinant human CFH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 24-349 aa of BC037285 Sequence: LPPRRNTEILTGSWSDQTYPEGTQAIYKCRPGYRSLGNVIMVCRKGEWVALNPLRKCQKRPCGHPGDTPFGTFTLTGGNVFEYGVKAVYTCNEGYQLLGEINYRECDTDGWTNDIPICEVVKCLPVTAPENGKIVSSAMEPDREYHFGQAVRFVCNSGYKIEGDEEMHCSDDGFWSKEKPKCVEISCKSPDVINGSPISQKIIYKENERFQYKCNMGYEYSERGDAVCTESGWRPLPSCEEKSCDNPYIPNGDYSPLRIKHRTGDEITYQCRNGFYPATRGNTAKCTSTGWIPAPRCTLKPCDYPDIKHGGLYHENMRRPYFPVAV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | complement factor H |
| 别名 | CFH, ARMD4, ARMS1, Factor H, FH |
| 计算分子量 | 1231 aa, 139 kDa |
| 观测分子量 | 140-150 kDa |
| GenBank蛋白编号 | BC037285 |
| 基因名称 | Complement factor H |
| Gene ID (NCBI) | 3075 |
| 偶联类型 | HRP |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P08603 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Complement factor H (CFH) is an abundant plasma glycoprotein that regulates the function of the alternative complement pathway in the fluid phase and on cellular surfaces (PMID: 2963625). Factor H binds to C3b, accelerates the decay of the alternative pathway C3-convertase (C3bBb), and acts as a cofactor for the factor I-mediated proteolytic inactivation of C3b (PMID: 15163532). It plays a critical role in the homeostasis of the complement system in plasma and in the protection of bystander host cells and tissues from damage by complement activation. Abnormalities in factor H have been associated with renal disease (PMID: 18190458).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP Complement factor H antibody HRP-12748 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
