CoraLite® Plus 488-conjugated Ch-TOG Monoclonal antibody

Ch-TOG Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H5G1

Cat No : CL488-67631

Print datasheet

Synonyms

ch TOG, CHTOG, CKAP5, FLJ35359, KIAA0097, MSPS, TOG, TOGp



经过测试的应用

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67631-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL488-67631 targets Ch-TOG in IF applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications IF/ICC
Cited ApplicationsIF
Tested Reactivity Human, mouse, rat, pig
Immunogen Ch-TOG fusion protein Ag28770 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cytoskeleton associated protein 5
Synonyms ch TOG, CHTOG, CKAP5, FLJ35359, KIAA0097, MSPS, TOG, TOGp
Observed Molecular Weight 220-240 kDa
GenBank Accession NumberBC120869
Gene Symbol CKAP5
Gene ID (NCBI) 9793
RRIDAB_2919511
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14008
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ch-TOG (colonic hepatic tumor-overexpressed gene), also known as XMAP215 or CKAP5, is a microtubule polymerase which can promotes cytoplasmic microtubule nucleation and elongation. Through interacting with Aurora-A and TACC3, it plays a major role in organizing spindle poles.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Ch-TOG antibody CL488-67631Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

Science

The mechanism of acentrosomal spindle assembly in human oocytes

Authors - Tianyu Wu