验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HSC-T6 cells, 4T1 cells, NIH/3T3 cells, Jurkat cells, HepG2 cells, HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human colon cancer tissue |
Planning an IHC experiment? We recommend our IHCeasy CSE1L Ready-To-Use IHC Kit. CSE1L primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67306-1-Ig targets CSE1L in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 |
CatNo: Ag29131 Product name: Recombinant human CSE1L protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 627-971 aa of BC109313 Sequence: RITCKANPAAVVNFEEALFLVFTEILQNDVQEFIPYVFQVMSLLLETHKNDIPSSYMALFPHLLQPVLWERTGNIPALVRLLQAFLERGSNTIASAAADKIPGLLGVFQKLIASKANDHQGFYLLNSIIEHMPPESVDQYRKQIFILLFQRLQNSKTTKFIKSFLVFINLYCIKYGALALQEIFDGIQPKMFGMVLEKIIIPEIQKVSGNVEKKICAVGITKLLTECPPMMDTEYTKLWTPLLQSLIGLFELPEDDTIPDEEHFIDIEDTPGYQTAFSQLAFAGKKEHDPVGQMVNNPKIHLAQSLHKLSTACPGRVPSMVSTSLNAEALQYLQGYLQAASVTLL 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CSE1 chromosome segregation 1-like (yeast) |
| 别名 | CAS, CSE1, CSE1L, Exp2, Exportin 2, Importin alpha re exporter, XPO2 |
| 计算分子量 | 971 aa, 110 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC109313 |
| 基因名称 | CSE1L |
| Gene ID (NCBI) | 1434 |
| RRID | AB_2882568 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P55060 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CSE1L antibody 67306-1-Ig | Download protocol |
| IHC protocol for CSE1L antibody 67306-1-Ig | Download protocol |
| WB protocol for CSE1L antibody 67306-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
