验证数据展示
经过测试的应用
| Positive IF-P detected in | human liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66250 targets C-Reactive Protein/CRP in IF-P applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag9883 Product name: Recombinant human CRP protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-91 aa of BC020766 Sequence: MEKLLCFLVLTSLSHAFGQTDMSRKAFVFPKESDTSYVSLKAPLTKPLKAFTVCLHFYTELSSTRGPNVLNWRALKYEVQGEVFTKPQLWP 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | C-reactive protein, pentraxin-related |
| 别名 | CRP, PTX1, C-reactive protein(1-205), C-Reactive Protein, C reactive protein |
| 计算分子量 | 224 aa, 25 kDa |
| 观测分子量 | 27 kDa |
| GenBank蛋白编号 | BC020766 |
| 基因名称 | CRP |
| Gene ID (NCBI) | 1401 |
| RRID | AB_2883544 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P02741 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name of CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein that belongs to the pentraxin family of proteins. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 C-Reactive Protein/CRP antibody CL594-66250 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
