• Featured Product
  • KD/KO验证

C-Reactive Protein/CRP Monoclonal antibody

C-Reactive Protein/CRP Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66250-1-Ig

产品说明书

CloneNo. 3F10A11

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig and More (1)

应用

WB, IHC, IF-P, ELISA and More (1)

CRP, PTX1, C-reactive protein(1-205), C-Reactive Protein, C reactive protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inserum from mouse injected with bacteria, pig liver tissue, rat liver tissue, serum from mouse injected with bacteria tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver tissue
Planning an IHC experiment? We recommend our IHCeasy CRP Ready-To-Use IHC Kit. CRP primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66250-1-Ig targets C-Reactive Protein/CRP in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat, pig
文献引用反应性human, mouse, rat, monkey
免疫原 C-Reactive Protein/CRP fusion protein Ag9883 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 C-reactive protein, pentraxin-related
别名 CRP, PTX1, C-reactive protein(1-205), C-Reactive Protein, C reactive protein
计算分子量 224 aa, 25 kDa
观测分子量 27 kDa
GenBank蛋白编号BC020766
基因名称 CRP
Gene ID (NCBI) 1401
RRIDAB_2881638
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP02741
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name of CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein that belongs to the pentraxin family of proteins. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.

实验方案

Product Specific Protocols
WB protocol for C-Reactive Protein/CRP antibody 66250-1-IgDownload protocol
IHC protocol for C-Reactive Protein/CRP antibody 66250-1-IgDownload protocol
IF protocol for C-Reactive Protein/CRP antibody 66250-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Nat Commun

Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model

Authors - Mengyan Hu
humanIF

Front Immunol

Expression and clinical significance of interleukin-6 pathway in cholangiocarcinoma

Authors - Dongqing Gu
humanIHC

J Cell Mol Med

Proteomic studies in VWA1-related neuromyopathy allowed new pathophysiological insights and the definition of blood biomarkers

Authors - Mohammed Athamneh
mouseWB

Front Immunol

Binding of Macrophage Receptor MARCO, LDL, and LDLR to Disease-Associated Crystalline Structures.

Authors - Anika Alberts

Sci Rep

A label-free fiber optic SPR biosensor for specific detection of C-reactive protein.

Authors - Wenjia Wang
humanWB

Sci Rep

C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.

Authors - Anika Alberts
Loading...