验证数据展示
经过测试的应用
| Positive IF/ICC detected in | SH-SY5Y cells |
For other applications, we recommend the unconjugated version of this antibody, 68272-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68272 targets CRMP5 in IF/ICC applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat, pig, rabbit |
| 免疫原 | CRMP5 fusion protein Ag0783 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | dihydropyrimidinase-like 5 |
| 别名 | DPYSL5, 3F2D8, CRMP 5, CRMP3-associated molecule, CRMP-5 |
| 计算分子量 | 61 kDa |
| 观测分子量 | 61 kDa |
| GenBank蛋白编号 | BC002874 |
| 基因名称 | CRMP5 |
| Gene ID (NCBI) | 56896 |
| RRID | AB_3672986 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9BPU6 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Collapsin response mediator proteins (CRMPs) are a family consisting of five homologous cytosolic phosphoproteins (CRMP1-5). All five CRMPs are abundantly expressed in the developing brain and the adult nervous system. CRMP5 is phylogenetically divergent from the other four CRMPs, sharing only 50% homology; this protein plays an important role in modulating filopodial dynamics and, in turn, restricting neuronal growth cone development.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CRMP5 antibody CL488-68272 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |