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验证数据展示

  • WB analysis of pig brain using 68272-1-Ig

    WB analysis of pig brain using 68272-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 68272-1-Ig (DPYSL5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of rabbit cerebellum using 68272-1-Ig

    WB analysis of rabbit cerebellum using 68272-1-Ig

    rabbit cerebellum tissue were subjected to SDS PAGE followed by western blot with 68272-1-Ig (DPYSL5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat cerebellum using 68272-1-Ig

    WB analysis of rat cerebellum using 68272-1-Ig

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 68272-1-Ig (CRMP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

CRMP5 Monoclonal antibody

CRMP5 Monoclonal Antibody for WB, ELISA
Cat No. 68272-1-Ig

产品说明书

CloneNo. 3F2D8

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat, Pig, Rabbit

应用

WB, ELISA

别名

CRAM, CRMP 5, CRMP3 associated molecule, CRMP5, dihydropyrimidinase like 5, DPYSL5, DRP 5, FLJ45383, ULIP 6, Ulip6, UNC33 like phosphoprotein 6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inpig brain tissue, rabbit cerebellum tissue, rat cerebellum tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68272-1-Ig targets CRMP5 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, Mouse, Rat, Pig, Rabbit
免疫原

CatNo: Ag0783

Product name: Recombinant human DPYSL5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 265-564 aa of BC002874

Sequence: KVVLAETTTAHATLTGLHYYHQDWSHAAAYVTVPPLRLDTNTSTYLMSLLANDTLNIVASDHRPFTTKQKAMGKEDFTKIPHGVSGVQDRMSVIWERGVVGGKMDENRFVAVTSSNAAKLLNLYPRKGRIIPGADADVVVWDPEATKTISASTQVQGGDFNLYENMRCHGVPLVTISRGRVVYENGVFMCAEGTGKFCPLRSFPDTVYKKLVQREKTLKVRGVDRTPYLGDVAVVVHPGKKEMGTPLADTPTRPVTRHGGMRDLHESSFSLSGSQIDDHVPKRASARILAPPGGRSSGIW

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 dihydropyrimidinase-like 5
别名 CRAM, CRMP 5, CRMP3 associated molecule, CRMP5, dihydropyrimidinase like 5, DPYSL5, DRP 5, FLJ45383, ULIP 6, Ulip6, UNC33 like phosphoprotein 6
计算分子量 61 kDa
观测分子量 61 kDa
GenBank蛋白编号BC002874
基因名称 CRMP5
Gene ID (NCBI) 56896
RRIDAB_2935355
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9BPU6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Collapsin response mediator proteins (CRMPs) are a family consisting of five homologous cytosolic phosphoproteins (CRMP1-5). All five CRMPs are abundantly expressed in the developing brain and the adult nervous system. CRMP5 is phylogenetically divergent from the other four CRMPs, sharing only 50% homology; this protein plays an important role in modulating filopodial dynamics and, in turn, restricting neuronal growth cone development.

实验方案

Product Specific Protocols
WB protocol for CRMP5 antibody 68272-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols