验证数据展示
经过测试的应用
| Positive IF/ICC detected in | SH-SY5Y cells |
| Positive FC (Intra) detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68021 targets CRMP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat, pig, rabbit, chicken |
| 免疫原 |
CatNo: Ag30090 Product name: Recombinant human CRMP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 291-537 aa of BC000252 Sequence: WSKNWAKAAAFVTSPPLSPDPTTPDYLTSLLACGDLQVTGSGHCPYSTAQKAVGKDNFTLIPEGVNGIEERMTVVWDKAVATGKMDENQFVAVTSTNAAKIFNLYPRKGRIAVGSDADVVIWDPDKLKTITAKSHKSAVEYNIFEGMECHGSPLVVISQGKIVFEDGNINVNKGMGRFIPRKAFPEHLYQRVKIRNKVFGLQGVSRGMYDGPVYEVPATPKYATPAPSAKSSPSKHQPPPIRNLHQS 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | collapsin response mediator protein 1 |
| 别名 | 2F4G5, CRMP 1, CRMP-1, Dihydropyrimidinase-related protein 1, DPYSL1 |
| 计算分子量 | 62 kDa |
| 观测分子量 | 62 kDa |
| GenBank蛋白编号 | BC000252 |
| 基因名称 | CRMP1 |
| Gene ID (NCBI) | 1400 |
| RRID | AB_2923861 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q14194 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CRMP1 (Collapsin response mediator protein 1) is also named as DPYSL1, ULIP3, DRP-1 and belongs to the collapsin response mediator protein family, which are a family of five cytoplasmic proteins predominantly expressed in the developing nervous system. CRMP1, the predicted 62 kDa protein and its 74 kDa isoform corresponding to its N-terminal splice variant already described for embryonic, neonatal, and adult brain of the rat are evidenced in the cortex of adult mouse (PMID:15834957). This protein is involved in invasion, and aggressiveness of prolactin pituitary tumors.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CRMP1 antibody CL488-68021 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
