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验证数据展示

  • FC experiment of U2OS using CL488-68021

    FC experiment of U2OS using CL488-68021

    1X10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CRMP1 (CL488-68021, Clone:2F4G5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of SH-SY5Y using CL488-68021

    IF Staining of SH-SY5Y using CL488-68021

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 CRMP1 antibody (CL488-68021, Clone: 2F4G5 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated CRMP1 Monoclonal antibody

CRMP1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68021

产品说明书

CloneNo. 2F4G5

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig, rabbit, chicken

应用

IF/ICC, FC (Intra)

别名

DRP 1, DPYSL1, Dihydropyrimidinase-related protein 1, CRMP-1, CRMP 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inU2OS cells
For other applications, we recommend the unconjugated version of this antibody, 68021-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68021 targets CRMP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig, rabbit, chicken
免疫原 CRMP1 fusion protein Ag30090 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 collapsin response mediator protein 1
别名 DRP 1, DPYSL1, Dihydropyrimidinase-related protein 1, CRMP-1, CRMP 1
计算分子量 62 kDa
观测分子量62 kDa
GenBank蛋白编号BC000252
基因名称 CRMP1
Gene ID (NCBI) 1400
RRIDAB_2923861
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14194
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRMP1 (Collapsin response mediator protein 1) is also named as DPYSL1, ULIP3, DRP-1 and belongs to the collapsin response mediator protein family, which are a family of five cytoplasmic proteins predominantly expressed in the developing nervous system. CRMP1, the predicted 62 kDa protein and its 74 kDa isoform corresponding to its N-terminal splice variant already described for embryonic, neonatal, and adult brain of the rat are evidenced in the cortex of adult mouse (PMID:15834957). This protein is involved in invasion, and aggressiveness of prolactin pituitary tumors.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CRMP1 antibody CL488-68021Download protocol
Standard Protocols
Click here to view our Standard Protocols