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验证数据展示

  • WB analysis of mouse cerebellum using 68021-1-Ig

    WB analysis of mouse cerebellum using 68021-1-Ig

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 68021-1-Ig (CRMP1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 68021-1-Ig

    IHC staining of mouse brain using 68021-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68021-1-Ig (CRMP1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CRMP1 Monoclonal antibody

CRMP1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68021-1-Ig

产品说明书

CloneNo. 2F4G5

宿主/亚型

Mouse / IgG2a

种属反应性

Mouse, Rat, Rabbit, Pig, Chicken, Human

应用

WB, IHC, ELISA

别名

CRMP 1, CRMP1, DPYSL1, DRP 1, DRP1, ULIP 3, ULIP3, Unc 33 like phosphoprotein 3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse cerebellum tissue, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, chicken brain tissue, pig cerebellum tissue, rabbit cerebellum tissue, rat cerebellum tissue
Positive IHC detected inmouse brain tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68021-1-Ig targets CRMP1 in WB, IHC, ELISA applications and shows reactivity with Mouse, Rat, Rabbit, Pig, Chicken, Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Mouse, Rat, Rabbit, Pig, Chicken, Human
免疫原 CRMP1 fusion protein Ag30090 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 collapsin response mediator protein 1
别名 CRMP 1, CRMP1, DPYSL1, DRP 1, DRP1, ULIP 3, ULIP3, Unc 33 like phosphoprotein 3
计算分子量 62 kDa
观测分子量62 kDa
GenBank蛋白编号BC000252
基因名称 CRMP1
Gene ID (NCBI) 1400
RRIDAB_2918766
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14194
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRMP1 (Collapsin response mediator protein 1) is also named as DPYSL1, ULIP3, DRP-1 and belongs to the collapsin response mediator protein family, which are a family of five cytoplasmic proteins predominantly expressed in the developing nervous system. CRMP1, the predicted 62 kDa protein and its 74 kDa isoform corresponding to its N-terminal splice variant already described for embryonic, neonatal, and adult brain of the rat are evidenced in the cortex of adult mouse (PMID:15834957). This protein is involved in invasion, and aggressiveness of prolactin pituitary tumors.

实验方案

Product Specific Protocols
WB protocol for CRMP1 antibody 68021-1-IgDownload protocol
IHC protocol for CRMP1 antibody 68021-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols