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验证数据展示

  • IF Staining of MCF-7 using CL594-66597

    IF Staining of MCF-7 using CL594-66597

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 CHCHD6 antibody (CL594-66597, Clone: 2A11E9 ) at dilution of 1:400.

  • FC experiment of A431 using CL594-66597

    FC experiment of A431 using CL594-66597

    1x10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated CHCHD6 Monoclonal antibody (CL594-66597, Clone:2A11E9)(red), or 0.4 ug CoraLite®594 Mouse IgG2a isotype control (11A1B2) (CL594-66360-2, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • FC experiment of MCF-7 using CL594-66597

    FC experiment of MCF-7 using CL594-66597

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated CHCHD6 Monoclonal antibody (CL594-66597, Clone:2A11E9)(red), or 0.4 ug CoraLite®594 Mouse IgG2a isotype control (11A1B2) (CL594-66360-2, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite®594-conjugated CHCHD6 Monoclonal antibody

CHCHD6 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66597

产品说明书

CloneNo. 2A11E9

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

2A11E9, CHCM1, Coiled-coil-helix cristae morphology protein 1, Coiled-coil-helix-coiled-coil-helix domain-containing protein 6, MIC25

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inA431 cells, MCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 66597-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66597 targets CHCHD6 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag14700

Product name: Recombinant human CHCHD6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-235 aa of BC006123

Sequence: MGSTESSEGRRVSFGVDEEERVRVLQGVRLSENVVNRMKEPSSPPPAPTSSTFGLQDGNLRAPHKESTLPRSGSSGGQQPSGMKEGVKRYEQEHAAIQDKLFQVAKREREAATKHSKASLPTGEGSISHEEQKSVRLARELESREAELRRRDTFYKEQLERIERKNAEMYKLSSEQFHEAASKMESTIKPRRVEPVCSGLQAQILHCYRDRPHEVLLCSDLVKAYQRCVSAAHKG

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 coiled-coil-helix-coiled-coil-helix domain containing 6
别名 2A11E9, CHCM1, Coiled-coil-helix cristae morphology protein 1, Coiled-coil-helix-coiled-coil-helix domain-containing protein 6, MIC25
计算分子量 235 aa, 26 kDa
观测分子量 26-29 kDa
GenBank蛋白编号BC006123
基因名称 CHCHD6
Gene ID (NCBI) 84303
RRIDAB_3673505
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9BRQ6
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL594 CHCHD6 antibody CL594-66597Download protocol
IF protocol for CL594 CHCHD6 antibody CL594-66597Download protocol
Standard Protocols
Click here to view our Standard Protocols
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