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验证数据展示

  • WB analysis using 66597-1-Ig

    WB analysis using 66597-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66597-1-Ig (CHCHD6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 66597-1-Ig

    IF Staining of MCF-7 using 66597-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CHCHD6 antibody (66597-1-Ig, Clone: 2A11E9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

CHCHD6 Monoclonal antibody

CHCHD6 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66597-1-Ig

产品说明书

CloneNo. 2A11E9

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

CHCHD6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells, HEK-293 cells
Positive IF/ICC detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66597-1-Ig targets CHCHD6 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 CHCHD6 fusion protein Ag14700 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 coiled-coil-helix-coiled-coil-helix domain containing 6
别名 CHCHD6
计算分子量 235 aa, 26 kDa
观测分子量 26-29 kDa
GenBank蛋白编号BC006123
基因名称 CHCHD6
Gene ID (NCBI) 84303
RRIDAB_2881957
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BRQ6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CHCHD6 antibody 66597-1-IgDownload protocol
IF protocol for CHCHD6 antibody 66597-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols