验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells |
For other applications, we recommend the unconjugated version of this antibody, 66788-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66788 targets Properdin/PFC in WB applications and shows reactivity with human, rat, pig samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | human, rat, pig |
| 免疫原 | Properdin/PFC fusion protein Ag9798 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | complement factor properdin |
| 别名 | CFP, PFC, complement factor properdin, Complement factor P, BFD |
| 计算分子量 | 469 aa, 51 kDa |
| 观测分子量 | 50 kDa, 100 kDa, 150 kDa |
| GenBank蛋白编号 | BC015756 |
| 基因名称 | CFP |
| Gene ID (NCBI) | 5199 |
| RRID | AB_3084274 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P27918 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CFP, also named as Properdin or CFP, is a 469 amino acid protein, which contains 7 TSP type-1 domains. CFP as secreted protein is a positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. Properdin deficiency poses a significant risk for severe meningococcal infections (PMID: 22229731). Properdin may be novel biomarker for future risk of type 2 diabetes (PMID: 22338105). In plasma, properdin exists as dimers, trimers or tetramers.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 Properdin/PFC antibody CL488-66788 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |