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CFP Monoclonal antibody

CFP Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D5A9

Cat No : 66788-1-Ig

Print datasheet

Synonyms

BFD, CFP, Complement factor P, complement factor properdin, PFC, PFD, PROPERDIN

验证数据展示

  • WB analysis of Jurkat using 66788-1-Ig

    WB analysis of Jurkat using 66788-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66788-1-Ig (CFP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inJurkat cells, human plasma
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66788-1-Ig targets CFP in WB, ELISA applications and shows reactivity with Human, pig, rat samples.

Tested Applications WB, ELISA
Tested Reactivity Human, pig, rat
Immunogen CFP fusion protein Ag9798 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name complement factor properdin
Synonyms BFD, CFP, Complement factor P, complement factor properdin, PFC, PFD, PROPERDIN
Calculated Molecular Weight 469 aa, 51 kDa
Observed Molecular Weight 50 kDa, 100 kDa, 150 kDa
GenBank Accession NumberBC015756
Gene Symbol CFP
Gene ID (NCBI) 5199
RRIDAB_2882133
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP27918
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CFP, also named as Properdin or CFP, is a 469 amino acid protein, which contains 7 TSP type-1 domains. CFP as secreted protein is a positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. Properdin deficiency poses a significant risk for severe meningococcal infections (PMID: 22229731). Properdin may be novel biomarker for future risk of type 2 diabetes (PMID: 22338105). In plasma, properdin exists as dimers, trimers or tetramers.

实验方案

Product Specific Protocols
WB protocol for CFP antibody 66788-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols