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验证数据展示

  • ChIP experiment of NIH/3T3 using 83791-6-RR (same clone as 83791-6-PBS)

    ChIP experiment of NIH/3T3 using 83791-6-RR (same clone as 83791-6-PBS)

    Chromatin was prepared from NIH/3T3 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of CEBPB (83791-6-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 83791-6-PBS in a different storage buffer formulation.

  • WB analysis using 83791-6-RR (same clone as 83791-6-PBS)

    WB analysis using 83791-6-RR (same clone as 83791-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83791-6-RR (CEBPB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83791-6-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 83791-6-RR (same clone as 83791-6-PBS)

    IHC staining of human ovarian cancer using 83791-6-RR (same clone as 83791-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83791-6-RR (CEBPB antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83791-6-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CEBPB antibody included
  • IF Staining of HepG2 using 83791-6-RR (same clone as 83791-6-PBS)

    IF Staining of HepG2 using 83791-6-RR (same clone as 83791-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CEBPB antibody (83791-6-RR, Clone: 240836G6 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83791-6-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83791-6-RR (same clone as 83791-6-PBS)

    FC experiment of HeLa using 83791-6-RR (same clone as 83791-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CEBPB Recombinant antibody (83791-6-RR, Clone:240836G6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83791-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83791-6-RR

    Affinity and Kinetic Characterization of 83791-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83791-6-RR against Human CEBPB were performed. The affinity constant is 69.2 pM.

CEBPB Recombinant monoclonal antibody, PBS Only

CEBPB Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA, ChIP-qPCR
Cat No. 83791-6-PBS

产品说明书

CloneNo. 240836G6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), ELISA, ChIP-qPCR

别名

240836G6, C EBP beta, C/EBP beta, C/EBP related protein 2, C/EBPbeta

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CEBPB Ready-To-Use IHC Kit. CEBPB primary antibody included.

产品信息

83791-6-PBS targets CEBPB in WB, IHC, IF/ICC, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA, ChIP-qPCR Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag20073

Product name: Recombinant human CEBPB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-266 aa of BC007538

Sequence: MQRLVAWDPACLPLPPPPPAFKSMEVANFYYEADCLAAAYGGKAAPAAPPAARPGPRPPAGELGSIGDHERAIDFSPYLEPLGAPQAPAPATATDTFEAAPPAPAPAPASSGQHHDFLSDLFSDDYGGKNCKKPAEYGYVSLGRLGAAKGALHPGCFAPLHPPPPPPPPPAELKAEPGFEPADCKRKEEAGAPGGGAGMAAGFPYALRAYLGYQAVPSGSSGSLSTSSSSSPPGTPSPADAKAPPTACYAGAAPAPSQVKSKAKKT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CCAAT/enhancer binding protein (C/EBP), beta
别名 240836G6, C EBP beta, C/EBP beta, C/EBP related protein 2, C/EBPbeta
计算分子量 345 aa, 36 kDa
观测分子量42 kDa, 46 kDa
GenBank蛋白编号BC007538
基因名称 CEBPB
Gene ID (NCBI) 1051
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP17676
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCAAT/enhancer-binding protein beta (CEBPB), also known as LAP, is a important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. It specifically binds to an IL-1 response element in the IL-6 gene. CEBPb mRNAs possess alternative translation-initiation codons, which result in the formation of truncated forms of the protein.Three variants of CEBPBs have been detected: a 46 kDa full-length liver-enriched transcription-activating protein (LAP1), a 42 kDa LAP2 and a 20 kDa liver-enriched transcription-inhibitory protein (LIP). (PMID:18820298).

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