• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83791-6-RR

    WB analysis using 83791-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83791-6-RR (CEBPB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovarian cancer using 83791-6-RR

    IHC staining of human ovarian cancer using 83791-6-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83791-6-RR (CEBPB antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEBPB antibody included
  • IF Staining of HepG2 using 83791-6-RR

    IF Staining of HepG2 using 83791-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CEBPB antibody (83791-6-RR, Clone: 240836G6 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83791-6-RR

    FC experiment of HeLa using 83791-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CEBPB Recombinant antibody (83791-6-RR, Clone:240836G6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83791-6-RR

    Affinity and Kinetic Characterization of 83791-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83791-6-RR against Human CEBPB were performed. The affinity constant is 69.2 pM.

CEBPB Recombinant antibody

CEBPB Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83791-6-RR

产品说明书

CloneNo. 240836G6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

CCAAT/enhancer-binding protein beta, C/EBPbeta, C/EBP related protein 2, C/EBP beta, C EBP beta

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, A549 cells, U-87 MG cells, MKN-45 cells, MDA-MB-231 cells
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CEBPB Ready-To-Use IHC Kit. CEBPB primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83791-6-RR targets CEBPB in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 CEBPB fusion protein Ag20073 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CCAAT/enhancer binding protein (C/EBP), beta
别名 CCAAT/enhancer-binding protein beta, C/EBPbeta, C/EBP related protein 2, C/EBP beta, C EBP beta
计算分子量 345 aa, 36 kDa
观测分子量42 kDa, 46 kDa
GenBank蛋白编号BC007538
基因名称 CEBPB
Gene ID (NCBI) 1051
RRIDAB_3671382
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP17676
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCAAT/enhancer-binding protein beta (CEBPB), also known as LAP, is a important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. It specifically binds to an IL-1 response element in the IL-6 gene. CEBPb mRNAs possess alternative translation-initiation codons, which result in the formation of truncated forms of the protein.Three variants of CEBPBs have been detected: a 46 kDa full-length liver-enriched transcription-activating protein (LAP1), a 42 kDa LAP2 and a 20 kDa liver-enriched transcription-inhibitory protein (LIP). (PMID:18820298).

实验方案

Product Specific Protocols
WB protocol for CEBPB antibody 83791-6-RRDownload protocol
IHC protocol for CEBPB antibody 83791-6-RRDownload protocol
IF protocol for CEBPB antibody 83791-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols