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验证数据展示

  • FC experiment of human monocyte-derived mature dendritic cells using 65581-1-MR

    FC experiment of human monocyte-derived mature dendritic cells using 65581-1-MR

    1x10^6 human monocyte-derived mature dendritic cells were surface stained with 0.25 ug Anti-Human CD83 Mouse IgG2a Recombinant Antibody (65581-1-MR, Clone: HB15e) (red) or 0.25 ug Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • IF Staining of Raji using 65581-1-MR

    IF Staining of Raji using 65581-1-MR

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD83 antibody (65581-1-MR, Clone: HB15e ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

Anti-Human CD83 (HB15e) Mouse IgG2a Recombinant Antibody

CD83 Uni-rAbTM Recombinant Antibody for IF/ICC, FC
Cat No. 65581-1-MR

产品说明书

CloneNo. HB15e

宿主/亚型

Mouse / IgG2a

种属反应性

human, non-human primates

应用

IF/ICC, FC

别名

B cell activation protein, B-cell activation protein, BL11, CD83 antigen, CD83 molecule

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive IF/ICC detected inRaji cells
Positive FC detected inHuman monocyte-derived mature dendritic cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65581-1-MR targets CD83 in IF/ICC, FC applications and shows reactivity with human, non-human primates samples.

经测试应用 IF/ICC, FC Application Description
经测试反应性 human, non-human primates
免疫原 Human CD83-transfected Cos cells 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 CD83 molecule
别名 B cell activation protein, B-cell activation protein, BL11, CD83 antigen, CD83 molecule
计算分子量 205 aa, 23 kDa
GenBank蛋白编号BC030830
基因名称 CD83
Gene ID (NCBI) 9308
ENSEMBL Gene IDENSG00000112149
RRIDAB_3670333
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928).

实验方案

Product Specific Protocols
IF protocol for CD83 antibody 65581-1-MRDownload protocol
FC protocol for CD83 antibody 65581-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols