验证数据展示
经过测试的应用
| Positive FC detected in | human monocyte-derived mature dendritic cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
Biotin-65581 targets CD83 in FC applications and shows reactivity with human, non-human primates samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | human, non-human primates |
| 免疫原 | Human CD83-transfected Cos cells 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | CD83 molecule |
| 别名 | B cell activation protein, B-cell activation protein, BL11, CD83 antigen, CD83 molecule |
| 计算分子量 | 205 aa, 23 kDa |
| GenBank蛋白编号 | BC030830 |
| 基因名称 | CD83 |
| Gene ID (NCBI) | 9308 |
| ENSEMBL Gene ID | ENSG00000112149 |
| 偶联类型 | Biotin |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for Biotin CD83 antibody Biotin-65581 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |