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CoraLite®594-conjugated CD8a Monoclonal antibody

CD8a Monoclonal Antibody for IF/ICC,IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC,IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G2B10

Cat No : CL594-66868

Print datasheet

Synonyms

CD8, CD8A, CD8a molecule, Leu2, MAL, p32

验证数据展示

  • IF Staining of human colon cancer using CL594-66868

    IF Staining of human colon cancer using CL594-66868

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 CD8a antibody (CL594-66868, Clone: 1G2B10 ) at dilution of 1:200, CoraLite® Plus 488 pan-keratin antibody (CL488-26411, green), CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Magenta).

  • IF Staining of human tonsillitis using CL594-66868

    IF Staining of human tonsillitis using CL594-66868

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL594-66868 (CD8a antibody) at dilution of 1:50.

  • IF Staining of human tonsillitis using CL594-66868

    IF Staining of human tonsillitis using CL594-66868

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD8a antibody (CL594-66868, Clone: 1G2B10 ) at dilution of 1:400. DAPI (blue).

经过测试的应用

Positive IF detected inhuman appendicitis tissue, human tonsillitis tissue, human colon cancer tissue
Planning an IHC experiment? We recommend our IHCeasy CD8 Ready-To-Use IHC Kit. CD8 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66868-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66868 targets CD8a in IF applications and shows reactivity with Human samples.

Tested Applications IF/ICC,IF-P
Tested Reactivity Human
Immunogen CD8a fusion protein Ag11111 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD8a molecule
Synonyms CD8, CD8A, CD8a molecule, Leu2, MAL, p32
Calculated Molecular Weight 235 aa, 26 kDa
GenBank Accession NumberBC025715
Gene Symbol CD8A
Gene ID (NCBI) 925
ENSEMBL Gene IDENSG00000153563
RRIDAB_2883630
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP01732
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells. This antibody is raised against 23-183 aa of human CD8a, the alpha chain of CD8 molecule.

实验方案

Product Specific Protocols
IF protocol for CL594 CD8a antibody CL594-66868Download protocol
Standard Protocols
Click here to view our Standard Protocols