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验证数据展示

  • WB analysis of MOLT-4 using 66868-1-Ig

    WB analysis of MOLT-4 using 66868-1-Ig

    MOLT-4 cells were subjected to SDS PAGE followed by western blot with 66868-1-Ig (CD8a antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 66868-1-Ig

    IHC staining of human tonsillitis using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD8A antibody included
  • IHC staining of human tonsillitis using 66868-1-Ig

    IHC staining of human tonsillitis using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD8A antibody included
  • IF Staining of human tonsillitis using 66868-1-Ig

    IF Staining of human tonsillitis using 66868-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD8a antibody (66868-1-Ig, Clone: 1G2B10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 66868-1-Ig

    IF Staining of human tonsillitis using 66868-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD8a antibody (66868-1-Ig, Clone: 1G2B10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human spleen using 66868-1-Ig

    IHC staining of human spleen using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human appendicitis using 66868-1-Ig

    IHC staining of human appendicitis using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human tonsillitis using 66868-1-Ig

    IHC staining of human tonsillitis using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human lymphoma using 66868-1-Ig

    IHC staining of human lymphoma using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human lymphoma using 66868-1-Ig

    IHC staining of human lymphoma using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human lymphoma using 66868-1-Ig

    IHC staining of human lymphoma using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human tonsillitis using 66868-1-Ig

    IHC staining of human tonsillitis using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66868-1-Ig (CD8 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD8A antibody included
  • IHC staining of human appendicitis using 66868-1-Ig

    IHC staining of human appendicitis using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66868-1-Ig (CD8 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD8A antibody included
  • IHC staining of human colon using 66868-1-Ig

    IHC staining of human colon using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66868-1-Ig (CD8 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD8A antibody included
  • IHC staining of human lymphoma using 66868-1-Ig

    IHC staining of human lymphoma using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD8A antibody included
  • IHC staining of human lymphoma using 66868-1-Ig

    IHC staining of human lymphoma using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD8A antibody included
  • IHC staining of human lymphoma using 66868-1-Ig

    IHC staining of human lymphoma using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD8A antibody included
  • IHC staining of human lymphoma using 66868-1-Ig

    IHC staining of human lymphoma using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD8A antibody included
  • IHC staining of human lymphoma using 66868-1-Ig

    IHC staining of human lymphoma using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD8A antibody included
  • IHC staining of human tonsillitis using 66868-1-Ig

    IHC staining of human tonsillitis using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD8A antibody included
  • IHC staining of human appendicitis using 66868-1-Ig

    IHC staining of human appendicitis using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD8A antibody included

CD8a Monoclonal antibody

CD8a Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66868-1-Ig

产品说明书

CloneNo. 1G2B10
引用文献(330)

宿主/亚型

Mouse / IgG1

种属反应性

human and More (2)

应用

WB, IHC, IF-P, ELISA and More (1)

别名

CD8, 1G2B10, CD8a molecule, Leu2, MAL

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMOLT-4 cells, human peripheral blood leukocyte
Positive IHC detected inhuman tonsillitis tissue, human appendicitis tissue, human breast cancer tissue, human colon cancer tissue, human lung cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy CD8A Ready-To-Use IHC Kit. CD8A primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:10000-1:40000
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66868-1-Ig targets CD8a in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF, IF-P
经测试反应性 human
文献引用反应性human, pig, bovine
免疫原

CatNo: Ag11111

Product name: Recombinant human CD8A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 23-183 aa of BC025715

Sequence: QFRVSPLDRTWNLGETVELKCQVLLSNPTSGCSWLFQPRGAAASPTFLLYLSQNKPKAAEGLDTQRFSGKRLGDTFVLTLSDFRRENEGCYFCSALSNSIMYFSHFVPVFLPAKPTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDI

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CD8a molecule
别名 CD8, 1G2B10, CD8a molecule, Leu2, MAL
计算分子量 235 aa, 26 kDa
观测分子量 32 kDa
GenBank蛋白编号BC025715
基因名称 CD8A
Gene ID (NCBI) 925
ENSEMBL Gene IDENSG00000153563
RRIDAB_2882205
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP01732
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells. This antibody is raised against 23-183 aa of human CD8a, the alpha chain of CD8 molecule.

实验方案

Product Specific Protocols
IF protocol for CD8a antibody 66868-1-IgDownload protocol
IHC protocol for CD8a antibody 66868-1-IgDownload protocol
WB protocol for CD8a antibody 66868-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Signal Transduct Target Ther

Krüppel-like factor 4 downregulation during esophageal cancer formation facilitates immune evasion and impairs immunotherapy

Authors - Xiao Hu
View Article
human

Cancer Cell

Proteogenomic characterization of non-functional pancreatic neuroendocrine tumors unravels clinically relevant subgroups

Authors - Shunrong Ji
View Article
IF

Cancer Cell

Spatial determinants of antibody-drug conjugate SHR-A1811 efficacy in neoadjuvant treatment for HER2-positive breast cancer

Authors - Ding Ma
View Article
humanIHC

Mol Cancer

ACOX2 destabilizes the MRE11-RAD50-NBS1 complex and boosts anticancer immunity via the cGAS-STING pathway in clear cell renal cell carcinoma.

Authors - Shiqi Ye
View Article
IHC

Mol Cancer

The construction of modular universal chimeric antigen receptor T (MU-CAR-T) cells by covalent linkage of allogeneic T cells and various antibody fragments

Authors - Tao Chen
View Article
humanIF

Cell Metab

Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer

Authors - Yu Jiang
View Article
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