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Species Reactivity
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验证数据展示

  • FC experiment of human PBMCs using 98235-1-RR (same clone as 98235-1-PBS)

    FC experiment of human PBMCs using 98235-1-RR (same clone as 98235-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD300e Rabbit Recombinant Antibody (98235-1-RR, Clone: 242031C10) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG. Cells were co-stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed This data was developed using the same antibody clone with 98235-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98235-1-RR (same clone as 98235-1-PBS)

    FC experiment of human PBMCs using 98235-1-RR (same clone as 98235-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD300e Rabbit Recombinant Antibody (98235-1-RR, Clone: 242031C10) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98235-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98235-1-RR

    Affinity and Kinetic Characterization of 98235-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98235-1-RR against Human CD300e were performed. The affinity constant is 0.456 nM.

Anti-Human CD300e Rabbit Recombinant Antibody, PBS Only

CD300e Uni-rAbTM Recombinant Antibody for FC
Cat No. 98235-1-PBS

产品说明书

CloneNo. 242031C10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

CMRF35A5, CMRF35 A5, CLM2, CLM 2, CD300LE

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

98235-1-PBS targets CD300e in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD300e molecule
别名 CMRF35A5, CMRF35 A5, CLM2, CLM 2, CD300LE
计算分子量23 kDa
GenBank蛋白编号NM_181449.2
基因名称 CD300E
Gene ID (NCBI) 342510
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ496F6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD300e, also known as immune receptor expressed on myeloid cells 2 (IREM-2), CLM-2, or CMRF35-A5, is one of the CD300 family members which belong to the immunoglobulin (Ig) superfamily (PMID: 15557162; 20039296). CD300e is a type I transmembrane glycoprotein that contains an extracellular region comprising an Ig-like domain, a transmembrane region, and a short cytoplasmic tail (PMID: 15557162; 29358324). It is by monocytes and myeloid dendritic cells and transmits an immune-activating signal by interacting with DNAX-activating protein 12 (DAP12) (PMID: 29358324).