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验证数据展示

  • FC experiment of human PBMCs using 98235-1-RR

    FC experiment of human PBMCs using 98235-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD300e Rabbit Recombinant Antibody (98235-1-RR, Clone: 242031C10) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG. Cells were co-stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed

  • FC experiment of human PBMCs using 98235-1-RR

    FC experiment of human PBMCs using 98235-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD300e Rabbit Recombinant Antibody (98235-1-RR, Clone: 242031C10) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98235-1-RR

    Affinity and Kinetic Characterization of 98235-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98235-1-RR against Human CD300e were performed. The affinity constant is 0.456 nM.

Anti-Human CD300e Rabbit Recombinant Antibody

CD300e Uni-rAbTM Recombinant Antibody for FC
Cat No. 98235-1-RR

产品说明书

CloneNo. 242031C10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

CMRF35A5, CMRF35 A5, CLM2, CLM 2, CD300LE

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98235-1-RR targets CD300e in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD300e molecule
别名 CMRF35A5, CMRF35 A5, CLM2, CLM 2, CD300LE
计算分子量23 kDa
GenBank蛋白编号NM_181449.2
基因名称 CD300E
Gene ID (NCBI) 342510
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ496F6
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD300e, also known as immune receptor expressed on myeloid cells 2 (IREM-2), CLM-2, or CMRF35-A5, is one of the CD300 family members which belong to the immunoglobulin (Ig) superfamily (PMID: 15557162; 20039296). CD300e is a type I transmembrane glycoprotein that contains an extracellular region comprising an Ig-like domain, a transmembrane region, and a short cytoplasmic tail (PMID: 15557162; 29358324). It is by monocytes and myeloid dendritic cells and transmits an immune-activating signal by interacting with DNAX-activating protein 12 (DAP12) (PMID: 29358324).

实验方案

Product Specific Protocols
FC protocol for CD300e antibody 98235-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols