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验证数据展示

  • FC experiment of U2OS using CL488-67961

    FC experiment of U2OS using CL488-67961

    1X10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CCT6B (CL488-67961, Clone:2F11A9) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated CCT6B Monoclonal antibody

CCT6B Monoclonal Antibody for FC (Intra)
Cat No. CL488-67961

产品说明书

CloneNo. 2F11A9

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

FC (Intra)

别名

Chaperonin containing T-complex polypeptide 1 subunit 6B, CCT-zeta-like, CCT-zeta-2, Cctz2, CCTZ 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inU2OS cells
Positive FC detected inU2OS cells
For other applications, we recommend the unconjugated version of this antibody, 67961-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67961 targets CCT6B in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, Mouse, Rat
免疫原

CatNo: Ag31295

Product name: Recombinant human CCT6B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 231-530 aa of BC027591

Sequence: LICNVSLEYEKTEVNSGFFYKTAEEKEKLVKAERKFIEDRVQKIIDLKDKVCAQSNKGFVVINQKGIDPFSLDSLAKHGIVALRRAKRRNMERLSLACGGMAVNSFEDLTVDCLGHAGLVYEYTLGEEKFTFIEECVNPCSVTLLVKGPNKHTLTQVKDAIRDGLRAIKNAIEDGCMVPGAGAIEVAMAEALVTYKNSIKGRARLGVQAFADALLIIPKVLAQNAGYDPQETLVKVQAEHVESKQLVGVDLNTGEPMVAADAGVWDNYCVKKQLLHSCTVIATNILLVDEIMRAGMSSLK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 chaperonin containing TCP1, subunit 6B (zeta 2)
别名 Chaperonin containing T-complex polypeptide 1 subunit 6B, CCT-zeta-like, CCT-zeta-2, Cctz2, CCTZ 2
计算分子量 530 aa, 58 kDa
观测分子量53-58 kDa
GenBank蛋白编号BC027591
基因名称 CCT6B
Gene ID (NCBI) 10693
RRIDAB_2923840
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ92526
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCT6B, also named as CCT-zeta-2, TCP-1-zeta-like, CCT-zeta-like, Testis-specific Tcp20 and Testis-specific protein TSA303, belongs to the TCP-1 chaperonin family. CCT6B is a molecular chaperone which assists the folding of proteins upon ATP hydrolysis. It is known to play a role, in vitro, in the folding of actin and tubulin. CCT6B has 3 isoforms with the molecular mass of 53-58 kDa.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CCT6B antibody CL488-67961Download protocol
Standard Protocols
Click here to view our Standard Protocols