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CoraLite® Plus 488-conjugated CBFB Monoclonal antibody

CBFB Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D7F2

Cat No : CL488-67885

Print datasheet

Synonyms

CBF beta, CBFB, PEA2 beta, PEBP2 beta, PEBP2B

验证数据展示

  • FC experiment of K-562 using CL488-67885

    FC experiment of K-562 using CL488-67885

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CBFB (CL488-67885, Clone:1D7F2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inK-562 cells
For other applications, we recommend the unconjugated version of this antibody, 67885-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67885 targets CBFB in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra)
Tested Reactivity Human
Immunogen CBFB fusion protein Ag31183 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name core-binding factor, beta subunit
Synonyms CBF beta, CBFB, PEA2 beta, PEBP2 beta, PEBP2B
Calculated Molecular Weight 182 aa, 22 kDa
GenBank Accession NumberBC018509
Gene Symbol CBFB
Gene ID (NCBI) 865
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13951
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CBFB (core binding factor subunit β), forms the heterodimeric complex core-binding factor (CBF). As a critical transcription factor, CBFB is frequently mutated in breast cancer and considered to be of significance in the pathogenesis of cancer.(PMID: 29932212) The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters.) It has calculated molecular weight around 22kDa.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CBFB antibody CL488-67885Download protocol
Standard Protocols
Click here to view our Standard Protocols