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验证数据展示

  • WB analysis using 67885-1-Ig

    WB analysis using 67885-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67885-1-Ig (CBFB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • FC experiment of K-562 using 67885-1-Ig

    FC experiment of K-562 using 67885-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human CBFB (67885-1-Ig, Clone:1D7F2) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CBFB Monoclonal antibody

CBFB Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 67885-1-Ig

产品说明书

CloneNo. 1D7F2

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, FC (Intra), ELISA

别名

1D7F2, CBF beta, CBF-beta, Core-binding factor subunit beta, PEA2-beta

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, MOLT-4 cells, K-562 cells, HSC-T6 cells
Positive FC (Intra) detected inK-562 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67885-1-Ig targets CBFB in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag31183

Product name: Recombinant human CBFB protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-182 aa of BC018509

Sequence: MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEIAFVATGTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLQRLDGMGCLEFDEERAQQEDALAQQAFEEARRRTREFEDRDRSHREEMEVRVSQLLAVTGKKTTRP

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 core-binding factor, beta subunit
别名 1D7F2, CBF beta, CBF-beta, Core-binding factor subunit beta, PEA2-beta
计算分子量 182 aa, 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC018509
基因名称 CBFB
Gene ID (NCBI) 865
RRIDAB_2918642
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ13951
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CBFB (core binding factor subunit β), forms the heterodimeric complex core-binding factor (CBF). As a critical transcription factor, CBFB is frequently mutated in breast cancer and considered to be of significance in the pathogenesis of cancer.(PMID: 29932212) The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters.) It has calculated molecular weight around 22kDa.

实验方案

Product Specific Protocols
WB protocol for CBFB antibody 67885-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols