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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67885-1-Ig (same clone as 67885-1-PBS)

    WB analysis using 67885-1-Ig (same clone as 67885-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67885-1-Ig (CBFB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67885-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 67885-1-Ig (same clone as 67885-1-PBS)

    FC experiment of K-562 using 67885-1-Ig (same clone as 67885-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human CBFB (67885-1-Ig, Clone:1D7F2) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67885-1-PBS in a different storage buffer formulation.

CBFB Monoclonal antibody, PBS Only

CBFB Monoclonal Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 67885-1-PBS

产品说明书

CloneNo. 1D7F2

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, FC (Intra), Indirect ELISA

别名

1D7F2, CBF beta, CBF-beta, Core-binding factor subunit beta, PEA2-beta

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67885-1-PBS targets CBFB in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 CBFB fusion protein Ag31183 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 core-binding factor, beta subunit
别名 1D7F2, CBF beta, CBF-beta, Core-binding factor subunit beta, PEA2-beta
计算分子量 182 aa, 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC018509
基因名称 CBFB
Gene ID (NCBI) 865
RRIDAB_2918642
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ13951
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CBFB (core binding factor subunit β), forms the heterodimeric complex core-binding factor (CBF). As a critical transcription factor, CBFB is frequently mutated in breast cancer and considered to be of significance in the pathogenesis of cancer.(PMID: 29932212) The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters.) It has calculated molecular weight around 22kDa.