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CoraLite® Plus 488-conjugated CAP1 Monoclonal antibody

CAP1 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H3A10

Cat No : CL488-68207

Print datasheet

Synonyms

CAP, CAP 1, CAP1, CAP1 PEN

验证数据展示

  • IF Staining of HepG2 using CL488-68207

    IF Staining of HepG2 using CL488-68207

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 CAP1 antibody (CL488-68207, Clone: 2H3A10 ) at dilution of 1:200.

  • IF Staining of HepG2 using CL488-68207

    IF Staining of HepG2 using CL488-68207

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CAP1 antibody (CL488-68207, Clone: 2H3A10 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-68207

    FC experiment of HeLa using CL488-68207

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CAP1 (CL488-68207, Clone:2H3A10) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CAP1 Ready-To-Use IHC Kit. CAP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68207-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68207 targets CAP1 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, mouse, rat, pig, rabbit
Immunogen CAP1 fusion protein Ag8876 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CAP, adenylate cyclase-associated protein 1 (yeast)
Synonyms CAP, CAP 1, CAP1, CAP1 PEN
Calculated Molecular Weight 475 aa, 52 kDa
Observed Molecular Weight52 kDa
GenBank Accession NumberBC013963
Gene Symbol CAP1
Gene ID (NCBI) 10487
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ01518
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CAP1, also known as CAP, CAP1-PEN, belongs to the CAP family and contains 1 C-CAP/cofactor C-like domain. CAP1 binds to monomeric actin and regulates the actin cytoskeleton to control cell size, adhesion, and migration (PMID: 23737525). CAP1 also functions as a receptor for inflammatory cytokine resistin (PMID: 24606903). CAP1 is an abundant and ubiquitously expressed protein. Western blot analysis detected CAP1 at an apparent molecular mass of 52 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CAP1 antibody CL488-68207Download protocol
FC protocol for CL Plus 488 CAP1 antibody CL488-68207Download protocol
Standard Protocols
Click here to view our Standard Protocols