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验证数据展示

  • IF Staining of HeLa using CL555-66693

    IF Staining of HeLa using CL555-66693

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 CALD1 antibody (CL555-66693, Clone: 1E1C11 ) at dilution of 1:200.

  • FC experiment of HeLa using CL555-66693

    FC experiment of HeLa using CL555-66693

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®555 Anti-Human CALD1 (CL555-66693, Clone:1E1C11) (red), or 0.8 ug CoraLite® Plus 555 Mouse IgG2b Isotype Control (MPC-11) (CL555-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®555-conjugated CALD1 Monoclonal antibody

CALD1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL555-66693

产品说明书

CloneNo. 1E1C11

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

Caldesmon, CDM, caldesmon 1, CAD, 1E1C11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®555
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CALD1 Ready-To-Use IHC Kit. CALD1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66693-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-66693 targets CALD1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 CALD1 fusion protein Ag13676 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 caldesmon 1
别名 Caldesmon, CDM, caldesmon 1, CAD, 1E1C11
计算分子量 538 aa, 63 kDa
观测分子量 70 kDa~75 kDa
GenBank蛋白编号BC040354
基因名称 CALD1
Gene ID (NCBI) 800
RRIDAB_2919692
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ05682
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL555 CALD1 antibody CL555-66693Download protocol
Standard Protocols
Click here to view our Standard Protocols