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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated CALD1 Monoclonal antibody

CALD1 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1E1C11

Cat No : CL647-66693

Print datasheet

Synonyms

CAD, CALD1, Caldesmon, caldesmon 1, CDM, H CAD, L CAD, NAG22

验证数据展示

  • FC experiment of HeLa using CL647-66693

    FC experiment of HeLa using CL647-66693

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human CALD1 (CL647-66693, Clone:1E1C11) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL647-66693

    IF Staining of HepG2 using CL647-66693

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 647 CALD1 antibody (CL647-66693, Clone: 1E1C11 ) at dilution of 1:100.

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CALD1 Ready-To-Use IHC Kit. CALD1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66693-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-66693 targets CALD1 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human
Immunogen CALD1 fusion protein Ag13676 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name caldesmon 1
Synonyms CAD, CALD1, Caldesmon, caldesmon 1, CDM, H CAD, L CAD, NAG22
Calculated Molecular Weight 538 aa, 63 kDa
GenBank Accession NumberBC040354
Gene Symbol CALD1
Gene ID (NCBI) 800
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ05682
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 CALD1 antibody CL647-66693Download protocol
FC protocol for CL Plus 647 CALD1 antibody CL647-66693Download protocol
Standard Protocols
Click here to view our Standard Protocols