验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, NIH/3T3 cells, HSC-T6 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-80713 targets Beta Tubulin in WB applications and shows reactivity with human, mouse, rat, zebrafish samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | human, mouse, rat, zebrafish |
| 免疫原 |
CatNo: Ag0117 Product name: Recombinant human Tubulin-beta protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 44-259 aa of BC000748 Sequence: LERISVYYNEASSHKYVPRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKLATPTYGDLNHLVSATMSGVTTSLRFPGQLNADLRKLAVNMVP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | tubulin, beta 3 |
| 别名 | Tubulin beta 3 chain, Tubulin beta III, β III tubulin , βIII tubulin , 2O13 |
| 计算分子量 | 450 aa, 50 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC000748 |
| 基因名称 | TUBB3 |
| Gene ID (NCBI) | 10381 |
| RRID | AB_3086573 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 激发激光 | Red Laser (633 nm) |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q13509 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 Beta Tubulin antibody CL750-80713 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
