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验证数据展示

  • WB analysis using CL750-80713

    WB analysis using CL750-80713

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-80713 (Beta Tubulin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using CL750-80713

    WB analysis using CL750-80713

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-80713 (Beta Tubulin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

CoraLite® Plus 750-conjugated Beta Tubulin Recombinant monoclonal antibody

Beta Tubulin Recombinant Antibody for WB
Cat No. CL750-80713

产品说明书

CloneNo. 2O13

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, zebrafish

应用

WB

别名

Tubulin beta III, Tubulin beta 3 chain, Tubulin beta-4 chain, Tubulin beta-3 chain, TUBB4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 750
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, NIH/3T3 cells, HSC-T6 cells
Planning an IHC experiment? We recommend our IHCeasy TUBB3 Ready-To-Use IHC Kit. TUBB3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80713-1-PBS

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-80713 targets Beta Tubulin in WB applications and shows reactivity with human, mouse, rat, zebrafish samples.

经测试应用 WB Application Description
经测试反应性 human, mouse, rat, zebrafish
免疫原

CatNo: Ag0117

Product name: Recombinant human Tubulin-beta protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 44-259 aa of BC000748

Sequence: LERISVYYNEASSHKYVPRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKLATPTYGDLNHLVSATMSGVTTSLRFPGQLNADLRKLAVNMVP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tubulin, beta 3
别名 Tubulin beta III, Tubulin beta 3 chain, Tubulin beta-4 chain, Tubulin beta-3 chain, TUBB4
计算分子量 450 aa, 50 kDa
观测分子量50-55 kDa
GenBank蛋白编号BC000748
基因名称 TUBB3
Gene ID (NCBI) 10381
RRIDAB_3086573
偶联类型 CoraLite® Plus 750 Fluorescent Dye
最大激发/发射波长755 nm / 780 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13509
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 Beta Tubulin antibody CL750-80713Download protocol
Standard Protocols
Click here to view our Standard Protocols