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Beta Tubulin Recombinant antibody, PBS Only

Beta Tubulin Recombinant Antibody for WB,IP,Indirect ELISA,IF,FC

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat, zebrafish

Applications

WB,IP,Indirect ELISA,IF,FC

Conjugate

Unconjugated

CloneNo.

2O13

Cat No : 80713-1-PBS

Print datasheet

Synonyms

beta 4, beta tubulin, MC1R, TUBB, TUBB4, Tubulin beta 3 chain, Tubulin beta 4 chain, Tubulin beta III, tubulin, beta 3

验证数据展示

  • WB analysis using 80713-1-RR (same clone as 80713-1-PBS)

    WB analysis using 80713-1-RR (same clone as 80713-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 80713-1-RR (same clone as 80713-1-PBS)

    IHC staining of rat brain using 80713-1-RR (same clone as 80713-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TUBB3 antibody included
  • IF Staining of C2C12 using 80713-1-RR (same clone as 80713-1-PBS)

    IF Staining of C2C12 using 80713-1-RR (same clone as 80713-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 80713-1-RR (same clone as 80713-1-PBS)

    IHC staining of rat brain using 80713-1-RR (same clone as 80713-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TUBB3 antibody included
  • IP experiment of HEK-293 using 80713-1-RR (same clone as 80713-1-PBS)

    IP experiment of HEK-293 using 80713-1-RR (same clone as 80713-1-PBS)

    IP result of anti-Beta Tubulin (IP:80713-1-RR, 4ug; Detection:66240-1-Ig 1:10000) with HEK-293 cells lysate 1360 ug. This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 80713-1-RR (same clone as 80713-1-PBS)

    FC experiment of HEK-293 using 80713-1-RR (same clone as 80713-1-PBS)

    1X10^6 HEK-293 cells were intracellularly stained with 0.2 ug Anti-Human Beta Tubulin (80713-1-RR, Clone:2O13) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy TUBB3 Ready-To-Use IHC Kit. TUBB3 primary antibody included.

产品信息

80713-1-PBS targets Beta Tubulin in WB, IP, IF, FC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, zebrafish samples.

Tested Applications WB,IP,Indirect ELISA,IF,FC
Tested Reactivity Human, mouse, rat, zebrafish
Immunogen Beta Tubulin fusion protein Ag0117 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tubulin, beta 3
Synonyms beta 4, beta tubulin, MC1R, TUBB, TUBB4, Tubulin beta 3 chain, Tubulin beta 4 chain, Tubulin beta III, tubulin, beta 3
Calculated Molecular Weight 450 aa, 50 kDa
Observed Molecular Weight50-55 kDa
GenBank Accession NumberBC000748
Gene Symbol TUBB3
Gene ID (NCBI) 10381
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13509
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.