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验证数据展示

  • FC experiment of HepG2 using CL488-67860

    FC experiment of HepG2 using CL488-67860

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human BCLAF1 (CL488-67860, Clone:1D6G7) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated BCLAF1 Monoclonal antibody

BCLAF1 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67860

产品说明书

CloneNo. 1D6G7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

KIAA0164, Btf, BCLAF1 and THRAP3 family member 1, Bcl-2-associated transcription factor 1, 1D6G7

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy BCLAF1 Ready-To-Use IHC Kit. BCLAF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67860-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67860 targets BCLAF1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag25210

Product name: Recombinant human BCLAF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 881-920 aa of BC132780

Sequence: SGSSPKWTHDKYQGDGIVEDEEETMENNEEKKDRRKEEKE

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 BCL2-associated transcription factor 1
别名 KIAA0164, Btf, BCLAF1 and THRAP3 family member 1, Bcl-2-associated transcription factor 1, 1D6G7
计算分子量 920 aa, 106 kDa
观测分子量145 kDa
GenBank蛋白编号BC132780
基因名称 BCLAF1
Gene ID (NCBI) 9774
RRIDAB_2923808
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9NYF8
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCLF1, also named as Bcl-2-associated transcription factor 1, is a 920 amino acid protein, which Interacts with Bcl-2 related proteins, EMD, with the adenovirus E1B 19 kDa protein and with DNA. BCLF1 as a death-promoting transcriptional repressor may be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. The calculated molecular weight of BCLF1 is a 110 kDa, but the modified BCLF1 protein is about 140-150 kDa.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 BCLAF1 antibody CL488-67860Download protocol
Standard Protocols
Click here to view our Standard Protocols
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