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CoraLite®594-conjugated BCLAF1 Monoclonal antibody

BCLAF1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1D6G7

Cat No : CL594-67860

Print datasheet

Synonyms

BCLAF1, bK211L9.1, BTF, KIAA0164

验证数据展示

  • FC experiment of HepG2 using CL594-67860

    FC experiment of HepG2 using CL594-67860

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human BCLAF1 (CL594-67860, Clone:1D6G7) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy BCLAF1 Ready-To-Use IHC Kit. BCLAF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67860-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67860 targets BCLAF1 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra)
Tested Reactivity Human, mouse, rat
Immunogen BCLAF1 fusion protein Ag25210 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name BCL2-associated transcription factor 1
Synonyms BCLAF1, bK211L9.1, BTF, KIAA0164
Calculated Molecular Weight 920 aa, 106 kDa
Observed Molecular Weight145 kDa
GenBank Accession NumberBC132780
Gene Symbol BCLAF1
Gene ID (NCBI) 9774
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NYF8
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCLF1, also named as Bcl-2-associated transcription factor 1, is a 920 amino acid protein, which Interacts with Bcl-2 related proteins, EMD, with the adenovirus E1B 19 kDa protein and with DNA. BCLF1 as a death-promoting transcriptional repressor may be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. The calculated molecular weight of BCLF1 is a 110 kDa, but the modified BCLF1 protein is about 140-150 kDa.

实验方案

Product Specific Protocols
FC protocol for CL594 BCLAF1 antibody CL594-67860Download protocol
Standard Protocols
Click here to view our Standard Protocols