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验证数据展示

  • IF Staining of HepG2 using CL488-28549

    IF Staining of HepG2 using CL488-28549

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Alpha-2-antiplasmin antibody (CL488-28549) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-28549

    FC experiment of HepG2 using CL488-28549

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Alpha-2-Antiplasmin Polyclonal Antibody (CL488-28549)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite® Plus 488-conjugated Alpha 2-Antiplasmin Polyclonal antibody

Alpha 2-Antiplasmin Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-28549

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

Alpha-2-antiplasmin, Serpin F2, a2-antiplasmin, A2AP, AAP

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 28549-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-28549 targets Alpha 2-Antiplasmin in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag29670

Product name: Recombinant human SERPINF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 348-491 aa of BC031592

Sequence: VATLSQLGLQELFQAPDLRGISEQSLVVSGVQHQSTLELSEVGVEAAAATSIAMSRMSLSSFSVNRPFLFFIFEDTTGLPLFVGSVRNPNPSAPRELKEQQDSPGNKDFLQSLKGFPRGDKLFGPDLKLVPPMEEDYPQFGSPK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
别名 Alpha-2-antiplasmin, Serpin F2, a2-antiplasmin, A2AP, AAP
计算分子量 491 aa, 55 kDa
观测分子量55 kDa
GenBank蛋白编号BC031592
基因名称 Alpha-2-antiplasmin
Gene ID (NCBI) 5345
RRIDAB_3672829
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08697
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SERPINF2, also termed Alpha-2-antiplasmin(a2-AP), a member of the serpin family of serine protease inhibitors. SERPINF2 is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in SERPINF2 result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 Alpha 2-Antiplasmin antibody CL488-28549Download protocol
IF protocol for CL Plus 488 Alpha 2-Antiplasmin antibody CL488-28549Download protocol
Standard Protocols
Click here to view our Standard Protocols
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