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验证数据展示

  • WB analysis using 28549-1-AP

    WB analysis using 28549-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28549-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 28549-1-AP

    IHC staining of mouse liver using 28549-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28549-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 28549-1-AP

    IHC staining of mouse liver using 28549-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28549-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 28549-1-AP

    IF Staining of HepG2 using 28549-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha-2-antiplasmin antibody (28549-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of human plasma using 28549-1-AP

    WB analysis of human plasma using 28549-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 28549-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Alpha 2-Antiplasmin Polyclonal antibody

Alpha 2-Antiplasmin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28549-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Alpha-2-antiplasmin, Serpin F2, ALPHA 2 PI, Alpha 2 AP, Alpha 2 antiplasmin

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, THP-1 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28549-1-AP targets Alpha 2-Antiplasmin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag29670

Product name: Recombinant human SERPINF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 348-491 aa of BC031592

Sequence: VATLSQLGLQELFQAPDLRGISEQSLVVSGVQHQSTLELSEVGVEAAAATSIAMSRMSLSSFSVNRPFLFFIFEDTTGLPLFVGSVRNPNPSAPRELKEQQDSPGNKDFLQSLKGFPRGDKLFGPDLKLVPPMEEDYPQFGSPK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
别名 Alpha-2-antiplasmin, Serpin F2, ALPHA 2 PI, Alpha 2 AP, Alpha 2 antiplasmin
计算分子量 491 aa, 55 kDa
观测分子量55 kDa
GenBank蛋白编号BC031592
基因名称 Alpha-2-antiplasmin
Gene ID (NCBI) 5345
RRIDAB_2918174
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08697
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SERPINF2, also termed Alpha-2-antiplasmin(a2-AP), a member of the serpin family of serine protease inhibitors. SERPINF2 is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in SERPINF2 result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis.

实验方案

Product Specific Protocols
IF protocol for Alpha 2-Antiplasmin antibody 28549-1-APDownload protocol
IHC protocol for Alpha 2-Antiplasmin antibody 28549-1-APDownload protocol
WB protocol for Alpha 2-Antiplasmin antibody 28549-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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