• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • IF Staining of HepG2 using CL488-68425

    IF Staining of HepG2 using CL488-68425

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 ATP6V1H antibody (CL488-68425, Clone: 3G7B5 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated ATP6V1H Monoclonal antibody

ATP6V1H Monoclonal Antibody for IF/ICC
Cat No. CL488-68425

产品说明书

CloneNo. 3G7B5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

IF/ICC

别名

3G7B5, CGI 11, CGI-11, MSTP042, NBP1

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy® ATP6V1H Ready-To-Use IHC Kit. ATP6V1H primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68425-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68425 targets ATP6V1H in IF/ICC applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag10130

Product name: Recombinant human ATP6V1H protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 136-483 aa of BC025275

Sequence: FTVHMAARIIAKLAAWGKELMEGSDLNYYFNWIKTQLSSQKLRGSGVAVETGTVSSSDSSQYVQCVAGCLQLMLRVNEYRFAWVEADGVNCIMGVLSNKCGFQLQYQMIFSIWLLAFSPQMCEHLRRYNIIPVLSDILQESVKEKVTRIILAAFRNFLEKSTERETRQEYALAMIQCKVLKQLENLEQQKYDDEDISEDIKFLLEKLGESVQDLSSFDEYSSELKSGRLEWSPVHKSEKFWRENAVRLNEKNYELLKILTKLLEVSDDPQVLAVAAHDVGEYVRHYPRGKRVIEQLGGKQLVMNHMHHEDQQVRYNALLAVQKLMVHNWEYLGKQLQSEQPQTAAARS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H
别名 3G7B5, CGI 11, CGI-11, MSTP042, NBP1
计算分子量 483 aa, 56 kDa
观测分子量 50 kDa
GenBank蛋白编号BC025275
基因名称 ATP6V1H
Gene ID (NCBI) 51606
RRIDAB_3673013
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9UI12
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ATP6V1H antibody CL488-68425Download protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...