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验证数据展示

  • WB analysis using 68425-1-Ig

    WB analysis using 68425-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68425-1-Ig (ATP6V1H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 68425-1-Ig

    WB analysis using 68425-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68425-1-Ig (ATP6V1H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 68425-1-Ig

    IF Staining of HepG2 using 68425-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATP6V1H antibody (68425-1-Ig, Clone: 3G7B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

ATP6V1H Monoclonal antibody

ATP6V1H Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68425-1-Ig

产品说明书

CloneNo. 3G7B5

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, rabbit, pig

应用

WB, IF/ICC, ELISA

别名

ATP6V1H, CGI 11, MSTP042, NBP1, Nef binding protein 1, SFD, SFDalpha, SFDbeta, V ATPase 50/57 kDa subunits, V ATPase subunit H, V type proton ATPase subunit H, Vacuolar proton pump subunit H, VMA13

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inU2OS cells, pig brain tissue, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy® ATP6V1H Ready-To-Use IHC Kit. ATP6V1H primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68425-1-Ig targets ATP6V1H in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat, rabbit, pig
免疫原

CatNo: Ag10130

Product name: Recombinant human ATP6V1H protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 136-483 aa of BC025275

Sequence: FTVHMAARIIAKLAAWGKELMEGSDLNYYFNWIKTQLSSQKLRGSGVAVETGTVSSSDSSQYVQCVAGCLQLMLRVNEYRFAWVEADGVNCIMGVLSNKCGFQLQYQMIFSIWLLAFSPQMCEHLRRYNIIPVLSDILQESVKEKVTRIILAAFRNFLEKSTERETRQEYALAMIQCKVLKQLENLEQQKYDDEDISEDIKFLLEKLGESVQDLSSFDEYSSELKSGRLEWSPVHKSEKFWRENAVRLNEKNYELLKILTKLLEVSDDPQVLAVAAHDVGEYVRHYPRGKRVIEQLGGKQLVMNHMHHEDQQVRYNALLAVQKLMVHNWEYLGKQLQSEQPQTAAARS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H
别名 ATP6V1H, CGI 11, MSTP042, NBP1, Nef binding protein 1, SFD, SFDalpha, SFDbeta, V ATPase 50/57 kDa subunits, V ATPase subunit H, V type proton ATPase subunit H, Vacuolar proton pump subunit H, VMA13
计算分子量 483 aa, 56 kDa
观测分子量 50 kDa
GenBank蛋白编号BC025275
基因名称 ATP6V1H
Gene ID (NCBI) 51606
RRIDAB_2943428
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9UI12
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains.

实验方案

Product Specific Protocols
IF protocol for ATP6V1H antibody 68425-1-IgDownload protocol
WB protocol for ATP6V1H antibody 68425-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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