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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated ASS1 Monoclonal antibody

ASS1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1D1A11

Cat No : CL594-66036

Print datasheet

Synonyms

Argininosuccinate synthase, argininosuccinate synthetase 1, ASS, ASS1, Citrulline aspartate ligase, CTLN1

验证数据展示

  • IF Staining of MCF-7 using CL594-66036

    IF Staining of MCF-7 using CL594-66036

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CL594-66036 (ASS1 antibody) at dilution of 1:100.

  • IF Staining of MCF-7 using CL594-66036

    IF Staining of MCF-7 using CL594-66036

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594-conjugated ASS1 antibody (CL594-66036, Clone: 1D1A11 ) at dilution of 1:100, 488phalloidin (green).

经过测试的应用

Positive IF detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy ASS1 Ready-To-Use IHC Kit. ASS1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66036-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL594-66036 is ASS1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC
Tested Reactivity human, pig, mouse, rat
Immunogen ASS1 fusion protein Ag9314 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name argininosuccinate synthetase 1
Synonyms Argininosuccinate synthase, argininosuccinate synthetase 1, ASS, ASS1, Citrulline aspartate ligase, CTLN1
Calculated Molecular Weight 412 aa, 47 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC009243
Gene Symbol ASS1
Gene ID (NCBI) 445
RRIDAB_2883485
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP00966
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ASS1 is a rate-limiting enzyme in the urea cycle. Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype(PMID:19006241).

实验方案

Product Specific Protocols
IF protocol for CL594 ASS1 antibody CL594-66036Download protocol
Standard Protocols
Click here to view our Standard Protocols