验证数据展示
经过测试的应用
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy ASS1 Ready-To-Use IHC Kit. ASS1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66036-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66036 targets ASS1 in IF/ICC applications and shows reactivity with human, pig, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, pig, mouse, rat |
| 免疫原 | ASS1 fusion protein Ag9314 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | argininosuccinate synthetase 1 |
| 别名 | Argininosuccinate synthase, argininosuccinate synthetase 1, ASS, ASS1, Citrulline aspartate ligase, CTLN1 |
| 计算分子量 | 412 aa, 47 kDa |
| 观测分子量 | 40-45 kDa |
| GenBank蛋白编号 | BC009243 |
| 基因名称 | ASS1 |
| Gene ID (NCBI) | 445 |
| RRID | AB_2883233 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P00966 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ASS1 is a rate-limiting enzyme in the urea cycle. Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype(PMID:19006241).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ASS1 antibody CL488-66036 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |