验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-85402 targets AQP2 in IF-P applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag29931 Product name: Recombinant human AQP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 207-271 aa of NM_000486 Sequence: GPLVGAILGSLLYNYVLFPPAKSLSERLAVLKGLEPDTDWEEREVRRRQSVELHSPQSLPRGTKA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | aquaporin 2 (collecting duct) |
| 别名 | Aquaporin 2, AQP-2, Aquaporin2 |
| 计算分子量 | 29 kDa |
| 观测分子量 | 28-29 kDa, 35-38 kDa |
| GenBank蛋白编号 | NM_000486 |
| 基因名称 | AQP2 |
| Gene ID (NCBI) | 359 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P41181 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AQP2 (Aquaporin2) is a water channel protein that is widely distributed among mammalian tissues and plays a major role in water homeostasis. AQP2 is mainly found in the apical cell membranes of the kidney's collecting duct cells and is critical for urinary concentration. AQP2 can be glycosylated and this antibody recognizes the 28-29 kDa non-glycosylated as well as the 35-40 kDa glycosylated forms of AQP2.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 AQP2 antibody CL647-85402 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
