验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse heart tissue |
| Positive FC (Intra) detected in | C2C12 cells |
Planning an IHC experiment? We recommend our IHCeasy ACTC1-specific Ready-To-Use IHC Kit. ACTC1-specific primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66125-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL594-66125 targets Alpha cardiac muscle actin specific in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | IF-P, FC (Intra) Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | actin, alpha, cardiac muscle 1 |
| 别名 | ACTC1-specific, ACTC1, Alpha actin, 1F2B9, ACTC |
| 计算分子量 | 42 kDa |
| GenBank蛋白编号 | NM_005159 |
| 基因名称 | ACTC1 |
| Gene ID (NCBI) | 70 |
| RRID | AB_2883519 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P68032 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms. The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL594 Alpha cardiac muscle actin specific antibody CL594-66125 | Download protocol |
| IF protocol for CL594 Alpha cardiac muscle actin specific antibody CL594-66125 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
