ACTC1-specific Monoclonal antibody

ACTC1-specific Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF-P, FC (Intra), ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1F2B9

Cat no : 66125-1-Ig

Print datasheet

Synonyms

ACTC1, Alpha actin, actin, ACTC1 cardiac muscle 1, ACTC 1



经过测试的应用

Positive WB detected inhuman heart tissue, pig heart tissue, rat heart tissue, mouse heart tissue
Positive IHC detected inhuman heart tissue, human heart and human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue
Positive FC (Intra) detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy ACTC1-specific Ready-To-Use IHC Kit. ACTC1-specific primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2500-1:10000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66125-1-Ig targets ACTC1-specific in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF-P, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, rabbit
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name actin, alpha, cardiac muscle 1
Synonyms ACTC1, Alpha actin, actin, ACTC1 cardiac muscle 1, ACTC 1
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberNM_005159
Gene Symbol ACTC1
Gene ID (NCBI) 70
RRIDAB_2881524
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP68032
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms.

实验方案

Product Specific Protocols
WB protocol for ACTC1-specific antibody 66125-1-IgDownload protocol
IHC protocol for ACTC1-specific antibody 66125-1-IgDownload protocol
IF protocol for ACTC1-specific antibody 66125-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Life Sci

Cadmium exposure induces a sex-dependent decline in left ventricular cardiac function

Authors - Michael L Fitch
WB

J Am Heart Assoc

Postinfarction Hearts Are Protected by Premature Senescent Cardiomyocytes Via GATA 4-Dependent CCN 1 Secretion.

Authors - Sumei Cui
humanIHC

Front Mol Biosci

Co-Overexpression of GRK5/ACTC1 Correlates With the Clinical Parameters and Poor Prognosis of Epithelial Ovarian Cancer.

Authors - Longyang Liu
humanWB

Immunol Res

The influences of α-hemolytic Streptococcus on class switching and complement activation of human tonsillar cells in IgA nephropathy.

Authors - Muyao Ye
mouseIF

Development

BMP4 triggers regulatory circuits specifying the cardiac mesoderm lineage

Authors - Pavel Tsaytler
humanIF

J Clin Invest

Frameshift variants in C10orf71 cause dilated cardiomyopathy in human, mouse, and organoid models

Authors - Yang Li