验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse skeletal muscle tissue |
For other applications, we recommend the unconjugated version of this antibody, 82714-5-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-82714-5 targets ACTA1-Specific in IF-P applications and shows reactivity with human, mouse, rat, pig, chicken samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | human, mouse, rat, pig, chicken |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | actin, alpha 1, skeletal muscle |
| 别名 | ACTA1, Alpha Actin, α actin, ACTA, ACTA1 skeletal Muscle Actin |
| 计算分子量 | 42 kDa |
| 观测分子量 | 39-42 kDa |
| GenBank蛋白编号 | NM_001100 |
| 基因名称 | ACTA1 |
| Gene ID (NCBI) | 58 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 激发激光 | Blue laser (488 nm) |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P68133 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ACTA1-Specific antibody CL488-82714-5 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
