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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82714-5-RR (same clone as 82714-5-PBS)

    WB analysis using 82714-5-RR (same clone as 82714-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82714-5-RR (ACTA1-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82714-5-PBS in a different storage buffer formulation.

  • IF Staining of mouse skeletal muscle using 82714-5-RR (same clone as 82714-5-PBS)

    IF Staining of mouse skeletal muscle using 82714-5-RR (same clone as 82714-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using ACTA1-Specific antibody (82714-5-RR, Clone: 5B15 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82714-5-PBS in a different storage buffer formulation.

  • IF Staining of mouse skeletal muscle using 82714-5-RR (same clone as 82714-5-PBS)

    IF Staining of mouse skeletal muscle using 82714-5-RR (same clone as 82714-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using ACTA1-Specific antibody (82714-5-RR, Clone: 5B15 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82714-5-PBS in a different storage buffer formulation.

  • IF Staining of mouse skeletal muscle using 82714-5-RR (same clone as 82714-5-PBS)

    IF Staining of mouse skeletal muscle using 82714-5-RR (same clone as 82714-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using ACTA1-Specific antibody (82714-5-RR, Clone: 5B15 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82714-5-PBS in a different storage buffer formulation.

ACTA1-Specific Recombinant antibody, PBS Only

ACTA1-Specific Uni-rAbTM Recombinant Antibody for WB, IF-P, Indirect ELISA
Cat No. 82714-5-PBS

产品说明书

CloneNo. 5B15

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig, chicken

应用

WB, IF-P, Indirect ELISA

别名

ACTA1, Alpha Actin, α actin, Actin, alpha skeletal muscle, Actin alpha skeletal muscle

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82714-5-PBS targets ACTA1-Specific in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, chicken samples.

经测试应用 WB, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, chicken
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 actin, alpha 1, skeletal muscle
别名 ACTA1, Alpha Actin, α actin, Actin, alpha skeletal muscle, Actin alpha skeletal muscle
计算分子量 42 kDa
观测分子量 39-42 kDa
GenBank蛋白编号NM_001100
基因名称 ACTA1
Gene ID (NCBI) 58
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP68133
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.