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验证数据展示

  • WB analysis using 82714-5-RR

    WB analysis using 82714-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82714-5-RR (ACTA1-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse skeletal muscle using 82714-5-RR

    IF Staining of mouse skeletal muscle using 82714-5-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using ACTA1-Specific antibody (82714-5-RR, Clone: 5B15 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of mouse skeletal muscle using 82714-5-RR

    IF Staining of mouse skeletal muscle using 82714-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using ACTA1-Specific antibody (82714-5-RR, Clone: 5B15 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse skeletal muscle using 82714-5-RR

    IF Staining of mouse skeletal muscle using 82714-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using ACTA1-Specific antibody (82714-5-RR, Clone: 5B15 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ACTA1-Specific Recombinant monoclonal antibody

ACTA1-Specific Uni-rAb® Recombinant Antibody for WB, IF-P, ELISA
Cat No. 82714-5-RR

产品说明书

CloneNo. 5B15

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig, chicken

应用

WB, IF-P, ELISA

别名

ACTA1, Alpha Actin, α actin, Actin, alpha skeletal muscle, Actin alpha skeletal muscle

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue, pig skeletal muscle tissue, chicken skeletal muscle tissue
Positive IF-P detected inmouse skeletal muscle tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82714-5-RR targets ACTA1-Specific in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, chicken samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, pig, chicken
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 actin, alpha 1, skeletal muscle
别名 ACTA1, Alpha Actin, α actin, Actin, alpha skeletal muscle, Actin alpha skeletal muscle
计算分子量 42 kDa
观测分子量 39-42 kDa
GenBank蛋白编号NM_001100
基因名称 ACTA1
Gene ID (NCBI) 58
RRIDAB_3086515
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP68133
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.

实验方案

Product Specific Protocols
IF protocol for ACTA1-Specific antibody 82714-5-RRDownload protocol
WB protocol for ACTA1-Specific antibody 82714-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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