Various lysates were subjected to SDS PAGE followed by western blot with 87054-1-RR (SAP30BP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87054-1-PBS in a different storage buffer formulation.

The mean SAP30BP concentration was determined to be 14.64 ng/mL in K-562 cell extract based on a 1.20 mg/mL extract load, 13.81 ng/mL in Jurkat cell extract based on a 1.20 mg/mL extract load, 9.67 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load, 11.54 ng/mL in HEK-293 cell extract based on a 1.20 mg/mL extract load, 7.34 ng/mL in HuH-7 cell extract based on a 1.20 mg/mL extract load, 10.99 ng/mL in HCT 116 cell extract based on a 1.20 mg/mL extract load and 4.93 ng/mL in BxPC-3 cell extract based on a 2.00 mg/mL extract load.

Sandwich ELISA standard curve of MP03025-1, Human SAP30BP Recombinant Matched Antibody Pair - PBS only. 87054-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag15051. 87054-3-PBS was HRP conjugated as the detection antibody. Range: 0.313-10 ng/mL.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SAP30BP antibody (87054-1-RR, Clone: 252137B1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87054-1-PBS in a different storage buffer formulation.

Biolayer interferometry (BLl) kinetic assays of 87054-1-RR against Human SAP30BP were performed. The affinity constant is 1.23 nM.