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验证数据展示

  • WB analysis using 87054-1-RR

    WB analysis using 87054-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87054-1-RR (SAP30BP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 87054-1-RR

    IF Staining of HeLa using 87054-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SAP30BP antibody (87054-1-RR, Clone: 252137B1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity of 87054-1-RR

    Affinity of 87054-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87054-1-RR against Human SAP30BP were performed. The affinity constant is 1.23 nM.

SAP30BP Recombinant monoclonal antibody

SAP30BP Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87054-1-RR

产品说明书

CloneNo. 252137B1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

HTRG, HTRP, SAP30-binding protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, A549 cells, Jurkat cells, HEK-293T cells, mouse spleen tissue, rat spleen tissue, mouse kidney tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87054-1-RR targets SAP30BP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag15051

Product name: Recombinant human SAP30BP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-308 aa of BC007592

Sequence: MAGKKNVLSSLAVYAEDSEPESDGEAGIEAVGSAAEEKGGLVSDAYGEDDFSRLGGDEDGYEEEEDENSRQSEDDDSETEKPEADDPKDNTEAEKRDPQELVASFSERVRNMSPDEIKIPPEPPGRCSNHLQDKIQKLYERKIKEGMDMNYIIQRKKEFRNPSIYEKLIQFCAIDELGTNYPKDMFDPHGWSEDSYYEALAKAQKIEMDKLEKAKKERTKIEFVTGTKKGTTTNATSTTTTTASTAVADAQKRKSKWDSAIPVTTIAQPTILTTTATLPAVVTVTTSASGSKTTVISAVGTIVKKAKQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SAP30 binding protein
别名 HTRG, HTRP, SAP30-binding protein
计算分子量 308 aa, 34 kDa
观测分子量45 kDa
GenBank蛋白编号BC007592
基因名称 SAP30BP
Gene ID (NCBI) 29115
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UHR5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SAP30BP, also named as SAP30-binding protein, is a 308 amino acid protein, which belongs to the HCNGP family. SAP30BP localizes in Nucleus and Interacts with SAP30. DDX54 Induces cell death and may act as a transcriptional corepressor of a gene related to cell survival. SAP30BP may be involved in the regulation of beta-2-microglobulin genes.

实验方案

Product Specific Protocols
IF protocol for SAP30BP antibody 87054-1-RRDownload protocol
WB protocol for SAP30BP antibody 87054-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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