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验证数据展示

  • WB analysis of human plasma using 86781-3-RR (same clone as 86781-3-PBS)

    WB analysis of human plasma using 86781-3-RR (same clone as 86781-3-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 86781-3-RR (AZGP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86781-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02669-2

    Sandwich ELISA sample test of MP02669-2

    Urine of six individual healthy human donors was measured. The ZAG/AZGP1 concentration of detected samples was determined to be 112.47 ng/mL with a range of 43.71-226.60 ng/mL.

  • Sandwich ELISA sample test of MP02669-2

    Sandwich ELISA sample test of MP02669-2

    Plasma of six individual healthy human donors was measured. The ZAG/AZGP1 concentration of detected samples was determined to be 137.18 ug/mL with a range of 49.90-221.70 ug/mL.

  • Sandwich ELISA standard curve of MP02669-2

    Sandwich ELISA standard curve of MP02669-2

    Sandwich ELISA standard curve of MP02669-2, Human ZAG/AZGP1 Recombinant Matched Antibody Pair - PBS only. 86781-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg6736. 86781-3-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • IHC staining of human breast cancer using 86781-3-RR (same clone as 86781-3-PBS)

    IHC staining of human breast cancer using 86781-3-RR (same clone as 86781-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86781-3-RR (Zinc Alpha 2 Glycoprotein antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86781-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AZGP1 antibody included
  • IHC staining of human breast cancer using 86781-3-RR (same clone as 86781-3-PBS)

    IHC staining of human breast cancer using 86781-3-RR (same clone as 86781-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86781-3-RR (Zinc Alpha 2 Glycoprotein antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86781-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AZGP1 antibody included
  • Affinity and Kinetic Characterization of 86781-3-RR

    Affinity and Kinetic Characterization of 86781-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86781-3-RR against Human Zinc Alpha 2 Glycoprotein were performed. The affinity constant is 63.7 pM.

Zinc Alpha 2 Glycoprotein Recombinant monoclonal antibody, PBS Only (Detector)

Zinc Alpha 2 Glycoprotein Uni-rAb® Recombinant Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA
Cat No. 86781-3-PBS

产品说明书

CloneNo. 251605B9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Sandwich ELISA, Indirect ELISA

别名

AZGP1, Zinc-alpha-2-glycoprotein, ZA2G, ZAG, Zn alpha 2 glycoprotein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy AZGP1 Ready-To-Use IHC Kit. AZGP1 primary antibody included.

产品信息

86781-3-PBS targets Zinc Alpha 2 Glycoprotein as part of a matched antibody pair:

MP02669-2: 86781-2-PBS capture and 86781-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg6736

Product name: Recombinant Human Zinc Alpha 2 Glycoprotein protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-N-rFc

Tag: N-rFc

Domain: 21-298 aa of BC033830

Sequence: QENQDGRYSLTYIYTGLSKHVEDVPAFQALGSLNDLQFFRYNSKDRKSQPMGLWRQVEGMEDWKQDSQLQKAREDIFMETLKDIVEYYNDSNGSHVLQGRFGCEIENNRSSGAFWKYYYDGKDYIEFNKEIPAWVPFDPAAQITKQKWEAEPVYVQRAKAYLEEECPATLRKYLKYSKNILDRQDPPSVVVTSHQAPGEKKKLKCLAYDFYPGKIDVHWTRAGEVQEPELRGDVLHNGNGTYQSWVVVAVPPQDTAPYSCHVQHSSLAQPLVVPWEAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 alpha-2-glycoprotein 1, zinc-binding
别名 AZGP1, Zinc-alpha-2-glycoprotein, ZA2G, ZAG, Zn alpha 2 glycoprotein
计算分子量 298 aa, 34 kDa
观测分子量 41 kDa
GenBank蛋白编号BC033830
基因名称 Zinc Alpha 2 Glycoprotein
Gene ID (NCBI) 563
RRIDAB_3745114
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP25311
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Zinc-alpha-2-glycoprotein (AZGP1) is a 41-kDa soluble protein normally found in body fluids, functions as a lipid mobilizing factor (PMID: 19188554). It is known to be expressed in the secretory epithelia of the liver, lung, breast, GI tract and sweat glands, sharing significant structural similarity with the class I major histocompatibility complex (MHC) antigens (PMID: 3422450). AZGP1 is involved in carcinogenesis and differentiation. Altered expression of AZGP1 has been reported in breast cancer, prostate cancer and lung adenocarcinoma, hepatocellular carcinoma, pancreatic carcinoma and oral tumors (PMID: 22625427).

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