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验证数据展示

  • WB analysis of human plasma using 86781-3-RR

    WB analysis of human plasma using 86781-3-RR

    human plasma were subjected to SDS PAGE followed by western blot with 86781-3-RR (AZGP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 86781-3-RR

    IHC staining of human breast cancer using 86781-3-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86781-3-RR (Zinc Alpha 2 Glycoprotein antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AZGP1 antibody included
  • IHC staining of human breast cancer using 86781-3-RR

    IHC staining of human breast cancer using 86781-3-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86781-3-RR (Zinc Alpha 2 Glycoprotein antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AZGP1 antibody included
  • Affinity and Kinetic Characterization of 86781-3-RR

    Affinity and Kinetic Characterization of 86781-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86781-3-RR against Human Zinc Alpha 2 Glycoprotein were performed. The affinity constant is 63.7 pM.

Zinc Alpha 2 Glycoprotein Recombinant monoclonal antibody

Zinc Alpha 2 Glycoprotein Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 86781-3-RR

产品说明书

CloneNo. 251605B9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

AZGP1, Zinc-alpha-2-glycoprotein, ZA2G, ZAG, Zn alpha 2 glycoprotein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman plasma
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy AZGP1 Ready-To-Use IHC Kit. AZGP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86781-3-RR targets Zinc Alpha 2 Glycoprotein in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg6736

Product name: Recombinant Human Zinc Alpha 2 Glycoprotein protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-N-rFc

Tag: N-rFc

Domain: 21-298 aa of BC033830

Sequence: QENQDGRYSLTYIYTGLSKHVEDVPAFQALGSLNDLQFFRYNSKDRKSQPMGLWRQVEGMEDWKQDSQLQKAREDIFMETLKDIVEYYNDSNGSHVLQGRFGCEIENNRSSGAFWKYYYDGKDYIEFNKEIPAWVPFDPAAQITKQKWEAEPVYVQRAKAYLEEECPATLRKYLKYSKNILDRQDPPSVVVTSHQAPGEKKKLKCLAYDFYPGKIDVHWTRAGEVQEPELRGDVLHNGNGTYQSWVVVAVPPQDTAPYSCHVQHSSLAQPLVVPWEAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 alpha-2-glycoprotein 1, zinc-binding
别名 AZGP1, Zinc-alpha-2-glycoprotein, ZA2G, ZAG, Zn alpha 2 glycoprotein
计算分子量 298 aa, 34 kDa
观测分子量 41 kDa
GenBank蛋白编号BC033830
基因名称 Zinc Alpha 2 Glycoprotein
Gene ID (NCBI) 563
RRIDAB_3745114
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP25311
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zinc-alpha-2-glycoprotein (AZGP1) is a 41-kDa soluble protein normally found in body fluids, functions as a lipid mobilizing factor (PMID: 19188554). It is known to be expressed in the secretory epithelia of the liver, lung, breast, GI tract and sweat glands, sharing significant structural similarity with the class I major histocompatibility complex (MHC) antigens (PMID: 3422450). AZGP1 is involved in carcinogenesis and differentiation. Altered expression of AZGP1 has been reported in breast cancer, prostate cancer and lung adenocarcinoma, hepatocellular carcinoma, pancreatic carcinoma and oral tumors (PMID: 22625427).

实验方案

Product Specific Protocols
IHC protocol for Zinc Alpha 2 Glycoprotein antibody 86781-3-RRDownload protocol
WB protocol for Zinc Alpha 2 Glycoprotein antibody 86781-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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